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13. | | MOREIRA, K. G.; SILVA, L. P.; BLOCH JÚNIOR, C. Miwaprin, a whey acidic protein-like in coral snake (Micrurus frontalis) venom. In: WORLD CONGRESS ON ANIMAL, PLANT AND MICROBIAL TOXINS, 15., 2006, Glasgow, Scotland. Abstact book... [S.l]: International Society on Toxinology, 2006. p. 259. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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14. | | MOREIRA, K. G.; SILVA, L. P.; BLOCH JÚNIOR, C. Phospholipases A2 from Micrurus frontalis venom. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | BRAND, G. D.; FURTADO JÚNIOR, J. R.; BLOCH JÚNIOR, C. Thermodynamics and kinetics of enzyme/inhibitors systems and interaction studies of peptides and DMPC vesicles. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | MAGALHÃES, M. T. Q.; BRAND, G. D.; BLOCH JÚNIOR, C.; GROSSI-de-SÁ, M. F. Análise da interação da toxina cry1la com as membranas do intestino médio (BBMVS) dos insetos Anthonomus grandis E Spodoptera frugiperda. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 10., 2005, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2005. p. 54. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | OLIVEIRA, N. B.; SCHWARTZ, C. A.; BLOCH JUNIOR, C.; PAULINO, L.; PIRES JÚNIOR, O. R. Bioacumulation of cyanotoxins in Hypophthalmichthys molitrix (Silver Carp) in Paranoá Lake, Brasilia-DF, Brazil. Bulletin of Environmental Contamination and Toxicology, v. 90, p. 308-313, 2013. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Instrumentação. |
Data corrente: |
20/04/2023 |
Data da última atualização: |
18/07/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 3 |
Autoria: |
PEREA, G. N. R.; PAVINATTO, A.; SCHNEIDER, R.; MUNK, M.; BRANDAO, H. de M.; CORREA, D. S. |
Afiliação: |
Universidade de São Paulo; Universidade Federal de São Carlos; Universidade Federal de Juiz de Fora; HUMBERTO DE MELLO BRANDAO, CNPGL; DANIEL SOUZA CORREA, CNPDIA. |
Título: |
Electrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin: investigation of morphological features, antibiotic release and antimicrobial properties. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Polymer Research, v. 30, 166, 2023. |
DOI: |
https://doi.org/10.1007/s10965-023-03535-7 |
Idioma: |
Inglês |
Conteúdo: |
Polymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus MenosPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides,... Mostrar Tudo |
Palavras-Chave: |
Antibiotic release; Electrospinning; Eletrofiação; Mastite; Slow-release. |
Thesagro: |
Antibiótico; Bovino; Doença Animal; Tratamento. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02915naa a2200301 a 4500 001 2153293 005 2023-07-18 008 2023 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1007/s10965-023-03535-7$2DOI 100 1 $aPEREA, G. N. R. 245 $aElectrospun nanofibers based on polyvinylpyrrolidone/chitosan and cloxacillin$binvestigation of morphological features, antibiotic release and antimicrobial properties.$h[electronic resource] 260 $c2023 520 $aPolymer nanofbers possess a range of attractive features that make them highly suitable for various biomedical application, including their high surface area/volume ratio, mechanical fexibility, porosity and potential for surface or bulk functionalization. Besides, such nanofbers can be used to encapsulate antibiotics to fabricate slow-release systems, but in this case the nanofbers should be fabricated with biocompatible and biodegradable polymers. Here we developed a novel nanocarrier system based on polyvinylpyrrolidone (PVP)/chitosan (CHI) electrospun nanofbers containing cloxacillin benzathine (CLO). PVP was chosen because of its ability to form nanofbers, while a small amount of chitosan was added to help control the CLO release, which antibiotic is widely employed to treat bacterial infections. The as-produced CLO-containing electrospun nanofbers were characterized regarding their morphological features and antibiotic release profle. SEM images revealed homogeneous and defectsfree nanofbers, exhibiting no discernible morphological variations between the CLO-containing and CLO-non-containing nanofbers. FTIR results indicate hydrogen bonds formation between carboxyl and carboxylate groups from CLO and hydroxyl groups from CHI. The antimicrobial properties of antibiotic-loaded nanofbers were investigated against Gram-positive bacteria S. aureus, yielding inhibition zone diameters of 12.5±1.6 mm and 29.8±1.3 mm for PVP/CHI/CLO and PVP/CLO nanofbers, respectively. Besides, the drug release kinetics study indicates that the nanocarrier system formed by PVP/CHI can release the antibiotics more slowly due to the chemical interactions between CHI and CLO compounds. Our results suggest that drug activity was preserved after encapsulation by electrospinning, and the diference in the inhibition zone diameter was corroborated by drug release mechanism experiments, indicating the system as a potential material to treat infections caused by S. aureus 650 $aAntibiótico 650 $aBovino 650 $aDoença Animal 650 $aTratamento 653 $aAntibiotic release 653 $aElectrospinning 653 $aEletrofiação 653 $aMastite 653 $aSlow-release 700 1 $aPAVINATTO, A. 700 1 $aSCHNEIDER, R. 700 1 $aMUNK, M. 700 1 $aBRANDAO, H. de M. 700 1 $aCORREA, D. S. 773 $tJournal of Polymer Research$gv. 30, 166, 2023.
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